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Promega 2'-deoxythymidine-5'-triphosphate (dttp)
2' Deoxythymidine 5' Triphosphate (Dttp), supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/2-deoxythymidine-5-triphosphate+dttp/2++deoxythymidine+5++triphosphate++dttp+/pm37930833-31-1-11
Average 90 stars, based on 1 article reviews
2'-deoxythymidine-5'-triphosphate (dttp) - by Bioz Stars, 2026-09
90/100 stars

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Reverse Transcription:

Article Title: Non-LTR-retroelement reverse transcriptase and uses thereof
Article Snippet: .. GsI-IIC RT (400 nM) was preincubated with the annealed R2/R2R-G heteroduplex (50 nM) and 50-nt acceptor template RNA (100 nM) in final 10 μl of reaction medium containing 200 mM NaCl, 5 mM MgCl2, 20 mM Tris-HCl pH 7.5, and 5 mM DTT for 30 min at room temperature, and the template-switching reverse transcription reactions were initiated by adding 0.4 μL of 25 mM dNTPs (an equimolar mix of 25 mM dATP, dCTP, dGTP, and dTTP, Promega). ..

Article Title: Direct actions of growth hormone in rainbow trout, Oncorhynchus mykiss, skeletal muscle cells in vitro.
Article Snippet: .. For each reaction, 2 μL of cDNA (from 400 ng RNA reverse transcription reaction) was combined with 2× GoTaq Green Master Mix (400 μM: dATP, dGTP, dCTP, dTTP; 3 mMMgCl2; Promega, Madison, WI, USA), 10 μMForward Primer, 10 μM Reverse Primer, and nuclease-free water. .. Each reaction was performed according to previous publications using the Bio-Rad T100 Thermocycler.

Article Title: Age at release affects developmental physiology and sex-specific phenotypic diversity of hatchery steelhead trout (Oncorhynchus mykiss).
Article Snippet: Total RNA was diluted with nuclease-free water (BioExpress, Radnor, PA) to 10.0 ng/μl and 30 ng per sample was reverse transcribed with Superscript II RNase H-reverse transcriptase (Invitrogen, Carlsbad, CA). .. Reverse transcription (RT) reaction conditions were as follows: 3.0 μl of 5X buffer, 1.5 μl of 0.1 M dTT, 0.75 μl of dNTPs (stock of 10 mM each dCTP, dGTP, dTTP and dATP; Promega, Madison WI), 0.225 μl random hexamers (500 ng/μl stock; Promega), 0.1875 μl Superscript II (200 U/μl; Invitrogen), 0.3 μl RNase inhibitor (20 U/μl; Promega), 6.0375 μl nuclease-free water (BioExpress) and 3.0 μl of template. .. RT reactions were conducted in a BioRad C1000 thermal cycler (BioRad, Hercules, CA) with the following temperature profile: 23 ̊C for 10 min, 48 ̊C for 60 min and 95 ̊C for 10 min, followed by a 4 ̊C incubation.

other:

Article Title: Plant miR6262 Modulates the Expression of Metabolic and Thermogenic Genes in Human Hepatocytes and Adipocytes
Article Snippet: Samples were incubated with Primer “random” (Roche, Basel, Switzerland) for 5 min at 70 °C. dNTP Mix (Set of dATP, dCTP, dGTP, dTTP; Promega), M-MLV-RT and Reaction Buffer from M-MLV-RT kit (Promega) were added to the samples and incubated for 60 min at 37 °C.

Article Title: Micropropagation and ISSR Molecular Analysis of the Endangered Species Sargassum fusiforme: A Biotechnological Approach
Article Snippet: Tissue culture is one of the most promising and practical methods for conserving endangered plant species.. Therefore, the present study evaluates the conservation of the endangered seaweed Sargassum fusiforme through tissue culturing techniques from different explants for the first time.. Besides, the genetic variation of the mother plant and cultivated explants using inter-simple sequence repeat (ISSR) techniques; this methodology was the first to be illustrated in such work for algae tissue culturing.

Article Title: MYC modulates TOP2A diffusion to promote substrate detection and activity
Article Snippet: Equal amounts of RNA resuspended in 13 ul H 2 O were mixed with 1 ul of 10 mM dNTP (10 mM each of dATP, dCTP, dGTP, dTTP; Promega, U1205, U1215, U1225, U1235) and 0.5 ul of random hexamers (Promega, C1181).

Amplification:

Article Title: Investigation of Azole Resistance Involving cyp 51A and cyp 51B Genes in Clinical Aspergillus flavus Isolates
Article Snippet: .. Amplification reactions were performed with a final volume of 50 μl containing 10 μl 5 × reaction buffer (pH 8.5), 25 mM MgCl 2 , 0.2 mM (each) dATP, dCTP, dGTP and dTTP (Promega, UK), 20 pmol of each primer, 2.5 U of GOTaq® DNA Polymerase (Promega, UK) and 400 ng of genomic DNA. .. PCR was performed in a thermocycler (Eppendorf, Germany), with the following amplification conditions: 94°C for 5 min, followed by 30 cycles of 30 s at 94°C, 30 s at 58°C and 1 min at 72°C and a final extension at 72°C for 10 min. PCR products were analyzed by agarose gel electrophoresis and purified using the Purification PCR Wizard® Kit (Promega, UK).

Recombinant:

Article Title: Cell wall modifications in Saccharomyces cerevisiae wine yeast through adaptive laboratory evolution with Tebuconazole
Article Snippet: Then the protocol of M-MLV RT (Moloney Murine Leukemia Virus Reverse Transcriptase) was used (Promega, USA). .. Ten μL de M-MLV RT 5X Reaction Buffer, 2.5 μL dNTPs (10 mM dATP, 10 mM dGTP, 10 mM dCTP y 10 mM dTTP) were added (Promega, USA), 1,2 μL Recombinant RNasin ® Ribonuclease Inhibitor (40 U/μL), 2 μL of enzyme M-MLV RT (200 U/μL) y 10.3 μL of Nuclease free water was added, with a final volume of 50 μL. ..



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